| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 81 | Holdemania filiformis | TGCAGGGCAACAATGACG | AGCGCACCGTGTTAAGCT | 58.65 | 59.97 | 183 | 90 |
100.00%
|
50.00%
|
| Primer 82 | Holdemania filiformis | CGGAAATCTGGGAAGCGCT | AGCGCACCGTGTTAAGCT | 60.45 | 59.97 | 228 | 90 |
100.00%
|
50.00%
|
| Primer 83 | Holdemania filiformis | GGTGTGCAGGGCAACAAT | AGCGCACCGTGTTAAGCT | 58.54 | 59.97 | 187 | 90 |
100.00%
|
50.00%
|
| Primer 84 | Holdemania filiformis | ATTTCCTGCAGGCCAGCT | TTCCGGATCCTCATGCGTG | 59.23 | 59.86 | 212 | 90 |
100.00%
|
50.00%
|
| Primer 85 | Holdemania filiformis | ACGGCAATGCTTCCAGGT | ATCGTGTTGCCGTCAGGT | 59.56 | 59.26 | 182 | 90 |
100.00%
|
50.00%
|
| Primer 86 | Holdemania filiformis | ACCGAATGCCTGTGCGAA | GCCGATTTCATACGCGACG | 59.97 | 59.80 | 276 | 90 |
100.00%
|
50.00%
|
| Primer 87 | Holdemania filiformis | AAGCGCTCGTCAGCCAAT | CGGCATTTGCGGAGTGATC | 60.05 | 59.65 | 197 | 90 |
100.00%
|
50.00%
|
| Primer 88 | Holdemania filiformis | AAGCGCTCGTCAGCCAAT | GCGGAGTGATCTGTGCGAA | 60.05 | 60.45 | 189 | 90 |
100.00%
|
50.00%
|
| Primer 89 | Holdemania filiformis | AAGCGCTCGTCAGCCAAT | CGGCATTTGCGGAGTGAT | 60.05 | 58.50 | 197 | 90 |
100.00%
|
50.00%
|
| Primer 90 | Holdemania filiformis | AGCGACGGCAATCGATGT | ACGATCGGCTTCAGCGAA | 59.82 | 59.43 | 153 | 90 |
100.00%
|
50.00%
|